Laboratory Medicine Updates - May 22, 2003
University of Virginia Health System
“Quality You Expect, Service You Deserve”
LABORATORY MEDICINE UPDATE
May 22, 2003
Qualitative Enterovirus Analysis by Real Time PCR Now Available
The Molecular Diagnostics Laboratory is now offering a real-time PCR assay for Enterovirus RNA detection. The procedure1 has been validated to detect RNA extracted from the following serotypes and is expected to work for other enteroviruses based on similarities in RNA sequences.
Coxsackievirus A9 and B5
Echovirus 1, 6, and 11
Test Name: Enterovirus by PCR (EVPCR)
MIS screen: CSF guide under “E”
CPT Codes: 83891, 83902, 83898, 83896x2, 83912
Sample Requirements: CSF only
Minimum Volume: 0.5 mL
Add-on Limitations: Specimens must be refrigerated after collection for storage less than 24 hours and frozen at -20oC for longer storage. Centrifuged specimens are not acceptable.
Availability and Turnaround Time: Monday through Friday, 1 working day
Reference Interval: None Detected
Please contact the Chemistry resident on-call (pic 1267) with any questions.
1Watkins-Riedel, T., Woegerbauer, M., Hollemann, D., Hufnagl, P. Rapid diagnosis of Enterovirus infections by real-time PCR on the LightCycler using the TaqMan format. Diagnostic Microbiology and Infectious Disease, 42, 99-105, 2002
Change in Reporting Units for High-Sensitivity C-Reactive Protein (hsCRP)
The hsCRP test is used to assess risk of cardiovascular
disease. To meet recently published
guidelines1 from the CDC and the American Heart
Association (AHA), results for hsCRP will be reported
in mg/L rather than mg/dL, increasing all
results by a factor of 10. The following risk
categories1 will be appended to each
result according to the CDC/AHA guidelines:
The recommendations include collecting two specimens two weeks apart and using the mean of the two results to assess risk. Any value over 10 mg/L should be investigated for potential underlying inflammatory disorder and not used for assessment of risk of cardiovascular disease.
Specimen type: Serum (gold-top).
Minimum volume: 1 mL whole blood (0.5 mL serum)
1Pearson, Mensah et al. Markers of Inflammation and Cardiovascular Disease: Application to Clinical and Public Health Practice. A Statement for Healthcare Professionals from the Centers for Disease Control and Prevention and the American Heart Association. Circulation 107:499-511, 2003.