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Deriving ES Cell Lines for Direct Gene Targeting onto the Desired Genetic Background. GTTF has derived multiple ES cell lines from several mouse strains, thus making it possible to improve the targeting efficiency with the C57BL/6 strain and to manipulate genes in strains, from which ES cells are not currently available. |
Gene Targeting with C57BL/6 Strain. GTTF has derived several C57BL/6 and C57BL/6J-Tyrc-2J ES cell lines. We injected black C57BL/6 ES cells into albino C57BL/6J-Tyrc-2J host blastocysts and created this male black albino chimera. |
Alkaline Phosphatase Assay to Characterize Derived ES Cell Lines. We used Vector® red alkaline phosphatase substrate kit to detect alkaline phosphatase activity present in the undifferentiated NZM2328 ES cells. |