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Gene Targeting and Transgenic Facility (GTTF)
Facility Description
About GTTF
Personnel & Labs
Advisory Committee
Track Record
Training Workshop
Poetry
Acknowledging GTTF
Gene Targeting
About Gene Targeting
Targeting Vector Design
Flowchart/Gene Targeting
Targeting Construct Request Form
Gene Targeting Request Form
MEF Request
ES Cell Request
Embryonic Stem Cell
About Embryonic Stem Cell
Karyotyping & Expansion
Derivation
Karyotyping Request Form
Expansion Request Form
Derivation Request Form
Genotyping Form
Transgenic Mouse
About Transgenic mice
Transgene Requirements
Flowchart/Transgenic Experiment
How to initiate injection
Transgene Detection
Transgene Injection Request Form
Chimeric Mouse
About ESC Injection
ESC Injection Request Form
From Chimera to Homozygous Null
Congenic Mouse
About Speed Congenics
From 129 to B6 Congenic
Genetic background
Congenic Request Form
Background Check Request Form
Linkage Analysis Request Form
Genotyping Request Form
Cryo & Rederivation
About Cryopreservation
Embryo Freezing
IVF
Embryo Isolation Request Form
Embryo Freezing Request Form
IVF Request Form
Embryo Transfer Request Form
Forms & Fees
All Forms & Fees
Transgene Injection Form
ESC Injection Form
Gene Targeting Form
Karyotyping Form
Speed congenic form
Embryo Freezing Form
Protocols
Transgene DNA prep
Transgene submission form
Primer testing
Copy Standard controls
Southern blots
DNA prep- screening
DNA prep-screen yolk sac
Mouse Information
Cre/Flox/Flp mice
Mouse strains used
Mouse husbandry
Transgene naming
Ear markings on mice
Mouse housing
Links to related sites
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Southern Blot protocols
Southern blots
Homologous recombination gene targeting -- screening strategies
Copy Standards, calculations
Gel and blot recipe/protocol
Radiolabeling using random priming
Example of high quality Southern blot with copy standard controls