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picture of GTTF

Protocols

Before project has been initiated

Transgene DNA preparation

Testing PCR primers or Southern blot conditions for transgene detection

Designing Southern blot strategy to detect homologous recombination gene targeting

Copy standards for Southern blot detection

Copy Standards for PCR detection of transgene

After project has been initiated

  • Tail DNA preparation
    • for Southern or PCR
  • Yolk sac DNA preparation
    • for Southern or PCR
  • DNA purification from yolk sacs, toes, or 2 mm tail
    • for PCR only
  • Southern blot protocols
  • Mouse strains, backcrossing
  • Mouse husbandry
  • Ear punch system to number mice

Last modified on: November 18, 2005

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Gene Targeting and Transgenic Facility (GTTF)

PO Box 800734

Charlottesville, VA 22908

434-982-3990

434-243-9393

fax: 434-982-1071

Maintained by wx8n@virginia.edu

© 2008 by the Rector and Visitors of the University of Virginia

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