Gene Targeting and Transgenic Facility Description
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The transgenic mouse has assumed increasing importance as an experimental system in recent years. Studies of normal gene function, altered gene expression, cis elements responsible for tissue-specific patterns of gene expression, and the production of animal models for human diseases are but some of the types of studies made possible by transgenic mouse technology. The Gene Targeting and Transgenic Facility (GTTF) provides a centralized service to efficiently produce transgenic mice for basic research for University of Virginia researchers and affiliated faculty.
Our facility is located within a microbiologic barrier in the new MR5 vivarium, so that mice supplied to you are as "clean" as those purchased from approved vendors, e.g. JAX.
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About Transgenic Mice |
| We microinject your DNA construct into the male pronucleus of the mouse zygote, and reimplant these into foster mothers to make transgenic mice. From one to several hundred copies of the transgene are integrated, usually at a single site and arranged in head to tail tandem arrays. Each transgenic founder animal will have the transgene integrated at a unique genomic site that may affect the expression of the transgene. We have made over 522 transgenic lines from many different constructs. We provide a guarantee of 3 founder animals per construct. |
To initiate service, fees
Transgene purification protocol
Transgene injection request form
Flow chart of transgenic experiment
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 The Transgenic Mouse Core Facility can begin a new gene targeting project by obtaining the genomic clone of the investigator's gene, characterizing it, and constructing the targeting construct.
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Track record of GTTF
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Alternatively, the TMCF can consult with investigators in the design of the targeting construct.and can begin with the investigator's targeting construct.
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Gene targeting information
Flow chart of gene targeting experiment
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 We microinject your targeted embryonic stem (ES) cells into the blastocoel of the 3.5 day mouse embryo and implant these into foster mothers to make chimeric mice. This is a crucial step in the production of mice carrying a targeted mutation. We have been very successful at producing chimeric mice giving high rates of germline transmission of targeted ES cells carrying the desired mutation. |
ES cell injection request form |
