Transgene Detection methods
Transgene detection:
- PCR primers should be designed to be transgene-specific, usually spanning
a novel promoter-gene junction.
- Southern blot digestions should be similarly designed to give a transgene-specific
band, distinguishable from possible endogenous bands if the gene is mouse or
mammal-derived.
 In this example,
the upper bands are endogenous mouse bands, and the lower band is transgene-specific.
Note that the endogenous bands are single-copy in intensity, and the transgene
band increases in intensity in proportion to how many copies are spiked into
the mouse genomic DNA.
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