Tissue Culture Facility

Mycoplasma Testing

  • $50.00 per assay
  • Requires 2 to 5 mls of conditioned culture media or a small growing culture
  • Must be at least 2 passages removed from antibiotic/antimycotic use
  • Please bring sample in a 15 ml centrifuge tube labeled with the following:  Investigator's name, phone number, name of cell line being tested.  Please use a marker that will not wash off in alcohol.
  • Tests are set up Monday and Thursday, with results available Friday and Monday.  Please bring samples no later than 10:30 am Monday or Thursday.  Samples dropped off after 10:30 will be held until the next testing day.
  • Please do not refrigerate or freeze samples.  This can cause problems with the assay.  Store samples in the incubator.
  • The test is an assay for mycoplasma driven conversion of 6-methylpurine deoxyribose to 6-methylpurine and 6-methylpurine riboside, both of which are toxic to mammalian cells.

 

* The Tissue Culture Facilty currently does not offer services outside of the University of Virginia community*