Tissue Culture Media and Reagents available from the Tissue Culture Facility
Invitrogen/Gibco products
|
Product |
Catalog number |
Unit |
Price |
Description |
|
Fetal Bovine Serum (FBS) |
16000-044 |
500 ml 50 ml |
$289.44 $28.94 |
Performance, mycoplasma, virus, bacteriophage, and endotoxin tested. United States origin. Certified |
| Newborn Calf Serum (NCS) | 16010-159 |
500 ml
|
$37.18 |
Performance, mycoplasma, virus, and endotoxin tested. Collected from calves 14 days old or less. |
| Horse Serum | 16050-122 | 500 ml |
$37.80 |
Performance, mycoplasma, endotoxin, and EIA tested (negative) serum from a donor herd. |
| DMEM | 11965-092 | 500 ml |
$4.63 |
Contains 4,500 mg/L D-glucose, L-glutamine, and pyridoxine hydrochloride, no sodium pyruvate. |
| DMEM | 11885-084 | 500 ml |
$5.10 |
Contains 1,000 mg/L D-glucose, L-glutamine, pyridoxine hydrochloride, and 110 mg/L sodium pyruvate |
|
DMEM |
11054-020 |
500 ml |
$8.54 |
Contains 1,000 mg/L D-glucose, pyridoxine hydrochloride, and 110 mg/L sodium pyruvate, but no L-glutamine or phenol red. |
| DMEM/F12 | 11320-033 | 500 ml |
$8.39 |
Contains L-glutamine and pyridoxine hydrochloride, but no HEPES buffer. |
| DMEM/F12 | 11330-032 | 500 ml |
$7.35 |
Contains 15 mM HEPES buffer, L-glutamine, and pyridoxine hydrochloride. |
| DMEM/F12 | 11039-021 | 500 ml |
$15.94 |
Contains 15 mM HEPES buffer, L-glutamine, and pyridoxine hydrochloride, but no phenol red. |
| F12 (Ham's) | 11765-054 | 500 ml |
$6.58 |
Contains L-glutamine |
| Grace's |
11605-094 |
500 ml |
$10.98 |
Contains 3,300 mg/L lactalbumin hydrolysate, 3,300 mg/L yeastolate, ans L-glutamine, but no insect hemolymph. Insect cell culture tested. |
| IMDM |
12440-053 |
500 ml 1,000 ml |
$7.98 $14.64 |
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no alpha-thioglycerol or 2-mercaptoethanol |
| McCoy's 5A Modified | 16600-082 | 500 ml | $12.06 | Contains L-Glutamine |
| Medium 199 | 11150-059 |
500 ml |
$6.49 |
Contains Earle's salts, L-glutamine, and 2,200 mg/L sodium bicarbonate. |
|
MEM |
11095-080 | 500 ml |
$5.08 |
Contains Earle's salts and L-glutamine. |
|
MEM |
10370-021 | 500 ml |
$6.40 |
Contains Earle's salts and non-essential amino acids, but no L-glutamine. |
|
Opti-MEM I |
31985-070 | 500 ml | $27.80 | Contains HEPES buffer, 2,400 mg/L Na Bicarb, hypoxanthine, thymidine, Na pyruvate, L-Glutamine, trace elements, growth factors, and phenol red reduced to 1.1 mg/L |
|
RPMI 1640 |
11875-093 | 500 ml |
$4.99 |
Contains L-glutamine. |
|
RPMI 1640 |
11835-030 | 500 ml |
$5.41 |
Contains L-glutamine but no phenol red. |
|
DPBS |
14190-144 | 500 ml |
$4.96 |
Contains no calcium or magnesium. |
|
DPBS (10x) |
14200-075 | 500 ml | $9.51 | Contains no calcium or magnesium. |
| Antibiotic/ Antimycotic | 15240-062 | 100 ml | $15.41 | Contains 10,000 units penicillin (base), 10,000 ug streptomycin (base), and 25 ug of amphotericin B/ml utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as FUNGIZONE (R) antimycotic in 0.85% saline |
| B-27 Serum -Free Supplement | 17504-044 | 10 ml | $59.30 | 50x liquid, serum-free supplement for the growth and long-term viability of hippocampal neurons. |
| HEPES buffer (1M) | 15630-080 | 100 ml | $41.83 | Contains 238.3 g/L prepared in distilled water. p.H. 7.2 - 7.5 |
| Fungizone | 15290-018 | 20 ml | $15.45 | Contains 250 ug amphotericin B and 250 ug sodium desoxycholate per ml as a solubilizer in distilled water. |
| L-glutamine | 25030-081 | 100 ml | $7.71 | Supplies at 29.2 mg/ml in 0.85% NaCl |
| MEM Non Essential Amino Acids | 11140-023 | 100 ml | $11.94 | 10 mM 100x solution prepared in distilled water. p.H. 1.5 to 1.7. |
| Pen/Strep | 15140-122 | 100 ml | $7.85 | Contains 10,000 units penicillin (base), 10,000 ug streptomycin (base)/ml utilizing penicillin G (sodium salt), streptomycin sulfate in 0.85% saline. |
| Sodium Pyruvate | 11360-070 | 100 ml | $6.61 | A 100x solution at 11,004 mg/L. |
| Sodium Bicarbonate | 25080-094 | 100 ml | $6.89 | A 7.5% weight/volume solution. |
| TrypLE Express | 12605-010 | 100 ml | $5.31 | A recombinant enzyme as an alternative to an animal trypsin for the dissoociation of adherent mammalian cells from plasticware. Stable at room temperature for 6 months. |
| Trypsin, 1x | 25300-054 | 100 ml | $4.72 | Porcine parvovirus and mycoplasma tested. Contains 0.5g/L of Trypsin (1:250) and 0.2g/L of EDTA·4Na in Hank’s Balanced Salt Solution without CaCl2, MgCl2·6H20 and MgSO4·7H20. |
| Trypsin, 10x | 15400-054 | 100 ml | $12.29 | Porcine parvovirus and mycoplasma tested. Contains 5.0 g/L of Trypsin (1:250) and 2.0 g/L of EDTA·4Na, 8.5 g/L NaCl, but no phenol red. |
|
Geneticin (G418) |
11811-023 11811-031 |
1 gram 5 gram |
$70.90 $194.10 |
Crystalline, non-sterile G418 sulfate. |
| Lipofectamine 2000 Transfection Reagent | 11668-027 | 0.75 ml | $247.38 | Exceptional transfection efficiency in a broad range of cell lines and high levels of recombinant protein expression. |
| Oligofectamine Transfection Reagent | 12252-011 | 1 ml | $225.27 | Forms stable complexes with oligos, permitting efficient transfection of oligos into eukaryotic cells |
| TOPO TA cloning kit (pCR 2.1 TOPO) | K4500-01 | 20 reactions | $412.14 | Contains linearized and topoisomerase I-activated pCR 2.1-TOPO vector, salt solution, dNTPs, control template and primers, M13 forward and reverse primers, One Shot Chemically Comp E. Coli cells, S.O.C. medium and Supercoiled control plasmid. |