University of Virginia Health System

HamRek Laboratory Protocols

Cloning:

• Ligations and Repair

Proteins:

• Polyacrylamide gels (SDS)

• Sample Preparation for SDS-PAGE

• Western blots
  • alkaline phosphatase
  • ECL
  • I-125 Protein A

Tissue Culture:

• Transfections
  • Ca-PO₄
  • DEAE - Dextran