TCV Surgery Lab Techniques

Our lab uses many surgical, cellular, and molecular techniques including:

Cell/Molecular Techniques:

Western blot analysis
ELISA
Multiplex, multi-cytokine assay
Immunohistochemistry
Cell culture
Real-time PCR
Affymetrix Gene-Chip Array
Northern/Southern blot
EMSA
Ribonuclease Protection Assay (RPA)        
Oxidative stress assays

Surgical/Other Techniques:

Transgenic/knockout mouse studies
Lung Histology & Morphometry
Lung broncho-alveolar lavage (BAL)
Rodent lung barium arteriogram and CT imaging
Bone marrow transplant in mice
Adoptive transfer of T cells into mice
Immune cell ablation in mice
Primary isolation and culture of various
       pulmonary immune and epithelial cells
Phase-contrast & fluorescent microscopy
Flow cytometry of lung and cultured cells