TCV Laboratory Resources:
FACILITIES: Our laboratories are located on the third floor of Building MR4. A total of 1800 square feet is available for the Division of Thoracic and Cardiovascular Surgery. This space includes a 750 sq. ft. fully equipped molecular biology laboratory (Room 3111), an adjacent 750 sq. ft. animal surgical laboratory (Room 3115) which contains dedicated small rodent surgery areas, a 200 sq. ft. equipment/animal-prep room, and a 100 sq. ft. cold room. The surgical laboratory houses our isolated rabbit lung model, isolated mouse lung system, surgery suites for pneumonectomy, and a rat model of cardio-pulmonary bypass. Autoclaving, laundry and dishwashing facilities are available on the same floor.
OFFICES: All of our offices are located across the hall from our laboratories. Room 3112 (200 sq. ft.) is the office of Dr. Laubach, Room 3116 (200 sq. ft.) is the office of Tony Herring, our Laboratory Manager, and Room 3118 (Room 3118, 400 sq. ft.)is the office for our PostDocs, Residents and Students. There is also a shared conference room (600 sq. ft.) located on our floor for laboratory meetings and presentations. In addition, there are desks in each laboratory for use by students and technicians.
COMPUTERS: All of our offices are equipped with laser printers and computers connected to the University-wide network for full internet access. Room 3118 has 3 Dell computers, Room 3116 has 2 Dell computers, Dr. Laubach's office has a Dell computer with laser printer, color printer and color scanner. Software on all computers includes MS Office, Adobe Photoshop, Adobe Acrobat, Endnote and SPSS. In addition, each laboratory has a Dell computer networked with our printers.
MAJOR EQUIPMENT: The molecular biology laboratory (Room 3111) is equipped with: 2.0GHz Pentium PC (network connected) connected to a Gel Documentation & Analysis System (Alpha Innotech's FlourChem 8900) and to a Nikon Digital camera attached to a Nikon phase-contrast microcope , refrigerated superspeed floor centrifuge (Sorvall RC5B), UVVIS-spectrophotometer (Pharmacia), Microquant UV-VIS ELISA plate reader (Bio-Tek), refrigerated bench-top swinging bucket centrifuge (IEC), two Eppendorf microcentrifuges, refrigerated Eppendorf microcentrifuge, PCR machine (MJ Research), Polytron tissue homogenizer (Brinkman), two upright -85°C freezers, cell-culture hood (Baker), two cell culture CO2 incubators (Forma), pH meter, two Eppendorf thermomixers, 3 water baths, two Mettler balances, inverted microscope (Olympus), bacterial culture oven incubator, shaker incubator, a dedicated space for radioactive hybridizations and related equipment, vacuum oven, UV crosslinker (Stratagene), vacuum concentrator (Labconco), gel dryer (BioRad), 4 BioRad power supplies, 3 BioRad Western blot apparatus, -20°C freezer, refrigerator, MilliQ water purification system, hybridization oven.
The animal surgery laboratory (Room 3115) is equipped with: an isolated mouse lung system with accompanying computer & ventilator (Hugo Sachs Elektronik, Germany), an isolated rabbit lung perfusion system with ventilator & computer, two Corning blood gas machines, refrigerator, -20°C freezer, 4 pressure-controlled small rodent ventilators (Kent Scientific), 2 small rodent surgery suites with heated platforms, Mettler balance, two operating microscopes (Leica), a Cytospin 3 centrifuge (ThermoShandon).