MOLECULAR & CELL BIOLOGY in the TCV RESEARCH LAB
A major part of our research involves the use of molecular and cell biology techniques to study mechanisms of lung growth, lung ischemia-reperfusion injury and spinal cord injury.Victor Laubach, PhD dedicates his time to teach and train Residents, Postdocs, students and other lab personnel various molecular biology techniques including: DNA/RNA/protein purification, Northern blot, Western blot, Southern blot, Enzyme Linked ImmunoSorbent Assay (ELISA), Electrophoretic Mobility Shift Assay (EMSA), Ribonuclease Protection Assay (RPA), Polymerase Chain Reaction (PCR), Immunohistochemistry, gene array, etc.
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Western blot showing upregulation of hepatoma-derived growth factor (HDGF) 7 days after pneumonectomy in lung. |
Protein array for angiogenesis-related proteins in mouse lung after pneumonectomy. |
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EMSA showing activation of NFkappaB in pig lung 30 and 60 minutes after transplant. |
Supershift analysis of NF-kB components. 
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We are using techniques to isolate primary endothelial and type 2 epithelial cells from mouse lungs to measure cell-specific expression of growth factors and receptors. The figure to the right illustrates expression of pro-SP-C (red staining) in isolated type 2 cells by immunohistochemistry, demonstrating >95% purity of this preparation. |
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Immunohistochemistry for BrdU, a marker of cell proliferation (brown stained nuclei), in the lung. 
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Immunohistochemistry of pig lung for BrdU, a marker of cell proliferation (blue stained nuclei). 
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Immunohistochemistry of mouse lung showing upregulation of HDGF (hepatoma-derived growth factor) after pneumonectomy. 
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Immunohistochemistry of mouse lung showing upregulation of HIMF (hypoxia induced mitogenic factor) after pneumonectomy. 
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In situ hybridization in mouse lung showing upregulation of transcripts for HIMF after pneumonectomy. 
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Immunohistochemistry for nitrotyrosine in mouse lung before (left) and after (right) ischemia-reperfusion injury.
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