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John  D.  Shannon
Degree(s): Ph.D.
Graduate School: University of Adelaide
Primary Appointment: Associate Professor of Research, Microbiology
Research Interests:
Protein primary structure determination, chromatography of biomolecules, biomolecule interactions.

Email Address: jds1c@virginia.edu
Research Description

My research revolves around several techniques in protein chemistry. As Director of the Protein Sciences Core in the Biomolecular Research Facility, the majority of my work is providing analyses for other investigators. One technique I use is Edman sequencing for analysis of the N-terminal sequence of proteins and peptides, commonly used to identify proteolysis sites and verify expression of proteins. Another technique I have used in a number of projects is chromatography (HPLC) to purify or analyze proteins, peptides and other molecules including nucleotides. Column types used are reverse phase, ion exchange and gel filtration. A relatively new technique in protein chemistry is surface plasmon resonance, used to measure interactions between an immobilized molecule and another which flows over it. The instrument can measure association and dissociation rates and binding constants. In addition I overseer a fluorescent gel scanner and analysis software, circular dichroism polarimeter and fluorescent plate reader. Research I have been involved in for some time is the structure and activity of snake venom metalloproteinases, in conjunction with Dr. Jay Fox and collaborators.

Selected Publications
  • Serrano, S.M.T., Kim, J., Wang, D., Dragulev, B., Shannon, J.D., Mann, H.H., Veit, G., Wagener, R., Koch, M. and Fox, J.W. (2006) “The cysteine-rich domain of snake venom metalloproteinases is a ligand for von Willebrand Factor A domains: Role in substrate targeting” J. Biol. Chem, 281: 39746-39756
  • Sullivan JT, Jeffery EF, Shannon JD, Ramakrishnan G. Characterization of the Siderophore of Francisella tularensis and Role of fslA in Siderophore Production. J Bacteriol. 2006 ;188(11):3785-95.
  • Serrano S.M., Jia L.G., Wang D., Shannon J.D., Fox J.W. 2005 “Function of the cysteine-rich domain of the haemorrhagic metalloproteinase atrolysin A: targeting adhesion proteins collagen I and von Willebrand factor.” Biochem. J. 391 69-76.
  • Neves-Ferreira AG. Perales J. Fox JW. Shannon JD. Makino DL. Garratt RC. Domont GB. “Structural and functional analyses of DM43, a snake venom metalloproteinase inhibitor from Didelphis marsupialis serum” (2002) J. Biol. Chem 277 13129-37
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    Contact Information
      Office Address: PO Box 800734, Jordan Hall, 232, 
      Office Phone: +1 434-243-9399, +1 434-924-2356
      Fax Phone: +1 434-982-2514
      Home Phone: +1 434-293-2953

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