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John  D.  Shannon
Degree(s): PhD
Graduate School: University of Adelaide
Primary Appointment: Associate Professor of Research, Microbiology, Immunology, and Cancer Biology
Research Interests:
Chromatography of biomolecules, protein interactions
Email Address: jds1c@virginia.edu
Research Description

My research revolves around several techniques in protein chemistry. As Director of the Shared Instrumentation Core in the Biomolecular Research Facility, the majority of my work is providing analyses for other investigators. The most common technique I use is chromatography (HPLC and FPLC) of proteins, peptides and other molecules including nucleotides. Chromatography isolates proteins and peptides, quantitates compounds, provides some information on purity, and gel filtration estimates size. Column types used are reverse phase, ion exchange and gel filtration. Another type of analysis I perform is surface plasmon resonance, used to measure interactions between an immobilized molecule and another which flows over it. The instrument can measure association and dissociation rates and binding constants. Currently I perform mass determinations of intact molecules in the Mass Spectrometry Core using MALDI-TOF. In addition I oversee a fluorescent plate reader, a fluorescent gel scanner and analysis software, circular dichroism polarimeter used to grain information on the secondary structure of proteins. Research I have been involved in for some time is the structure and activity of snake venom metalloproteinases and basement membrane proteins in conjunction with Dr. Jay Fox and collaborators.

Selected Publications
  • Chianese-Bullock KA. Lewis ST. Sherman NE. Shannon JD. Slingluff CL Jr. “Multi-peptide vaccines vialed as peptide mixtures can be stable reagents for use in peptide-based immune therapies.” Vaccine. 271764-17770, 2009.
  • Meller N. Westbrook MJ. Shannon JD. Guda C. Schwartz MA.(2008) Function of the N-terminus of zizimin1: autoinhibition and membrane targeting. Biochemical Journal. 409 525-33.
  • Serrano, S.M.T., Kim, J., Wang, D., Dragulev, B., Shannon, J.D., Mann, H.H., Veit, G., Wagener, R., Koch, M. and Fox, J.W. (2006) “The cysteine-rich domain of snake venom metalloproteinases is a ligand for von Willebrand Factor A domains: Role in substrate targeting” J. Biol. Chem, 281: 39746-39756.
  • Sullivan JT, Jeffery EF, Shannon JD, Ramakrishnan G. Characterization of the Siderophore of Francisella tularensis and Role of fslA in Siderophore Production. J Bacteriol. 2006 ;188(11):3785-95.
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    Contact Information
      Office Address: PO Box 800734 Jordan Hall, 232, 
      Office Phone: +1 434-243-9399, +1 434-924-2356
      Fax Phone: +1 434-982-2514
      Home Phone: +1 434-293-2953

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