James W. Mandell, M.D., Ph.D.

Associate Professor of Pathology 

EDUCATION:
Medical School: Cornell University Medical College, New York, NY.  MD, 1992; Ph.D, 1991 M.D./Ph.D.
 
Graduate School: Postdoctoral Neuroscience, University of Virginia, 1992 - 1994 

Residency: Anatomic Pathology, University of Virginia, 1994 - 1995
1995 - 1996  Chief Resident, Anatomic Pathology, University of Virginia

Fellowship: Fellow, Neuropathology, University of Virginia, 1996 - 1998  

CLINICAL:

Diagnostic neuropathology of neuromuscular disorders, neurodegenerative disease, nervous system neoplasms. See my neuromuscular pathology site.

RESEARCH:

  • Signal transduction mechanisms underlying the astroglial response to neuronal injury and degeneration
  • Transcriptional and post-transcriptional mechanisms responsible for persistence of reactive astroglial phenotypes

    Astrocytes play an active role in the central nervous system's response to many forms of neural injury and neurodegenerative disease. The underlying mechanisms and functional consequences of this cellular response are poorly understood. My laboratory has recently begun to investigate transmembrane signaling pathways important for the astroglial response to neuronal injury. Several lines of evidence support the role of MAP kinase pathways in the initiation and persistence of the reactive astrocytic phenotype. We utilize techniques of cellular and molecular neuroscience to elucidate the intracellular signal transduction mechanisms involved, as well the transcriptional and post-transcriptional machinery necessary for the persistence of reactive astrocytic phenotypes. Specific techniques include primary neuronal and glial culture and coculture, DNA transfection, promoter/reporter assays, immunocytochemistry, in situ hybridization, kinase/phosphorylation assays, and protein and nucleic acid blotting.

    More information on my research laboratory.

REFERENCES:

  • Correa-Cerro LS, Mandell JW. Molecular mechanisms of astrogliosis: new approaches with mouse genetics. J Neuropathol Exp Neurol. 2007 Mar;66(3):169-76. (Review.) 
  • Bourne TD, Mandell JW, Matsumoto JA., Jane JA, Lopes MB. Primary disseminated leptomeningeal oligodendroglioma with chromosome 1p deletion. J Neurosurg. 2006. 105:465-9. 
  • Hamilton DK, Bourne TD, Ahmed H, Cousar JB, Mandell JW, Sheehan JP.
    Follicular lymphoma of the dura: case report. Neurosurgery. 2006 Sep;59(3):E703 
  • McFarland KL, Wilkes, SR, Koss, SF, Ravichandran K, and Mandell JW. Neural-specific inactivation of ShcA in the nervous system causes neural progenitor apoptosis and microcephaly. J Neurosci. 2006 26:7885-97. 
  • Carbonell WS, Murase S, Horwitz AF, Mandell JW. Migration of perilesional microglia after focal brain injury and modulation by CC chemokine receptor 5: an in situ time-lapse confocal imaging study. J Neurosci. 2005 Jul 27;25(30):7040-7.
  • Carbonell WS, Murase SI, Horwitz AF, Mandell JW. Infiltrative microgliosis: activation and long-distance migration of subependymal microglia following periventricular insults. J Neuroinflammation. 2005 2:5.
  • Heffron D, Mandell JW. Differential localization of MAPK activated protein kinases RSK1 and MSK1 in mouse brain. Brain Res Mol Brain Res. 2005 May 20;136(1-2):134-41. Epub 2005 Feb 25.
  • Heffron DS, Mandell JW. Opposing roles of ERK and p38 MAP kinases in FGF2-induced astroglial process extension. Mol Cell Neurosci. 2005 Apr;28(4):779-90.
  • Heffron D and Mandell JW. Opposing roles of ERK and p38 MAP Kinases in FGF2-induced astroglial process extension. Mol Cell Neurosci. 2005 28:779-790
  • Carbonell WS, Murase SI, Horwitz AF, Mandell JW. Infiltrative microgliosis: activation and long-distance migration of subependymal microglia following periventricular insults. J Neuroinflammation. 2005 Jan 28;2(1):5.
  • Tountas NA, Mandell JW, Everett AD, Brautigan DL. Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells. Histochem Cell Biol. 2004 121:343-50
  • Mandell JW. Phosphorylation state-specific antibodies: applications in investigative and diagnostic pathology. Am J Pathol. 2003 63:1687-98.
  • Carbonell WS and Mandell JW. Transient Neuronal But Sustained Glial Activation of ERK/MAP Kinase following Focal Brain Injury in Mice. J Neurotrauma 2003 20:327-336.
  • Mandell JW, Manabe R, Horwitz AF, Baumgart JP. Fluorescence Imaging of Mobility Shifts: An Expression Cloning Method for Identification of Cell Signaling Targets. (Cover Article). Laboratory Investigation 2002 82:1631-36. 
  • Minassian BA, Aiyar R, Alic S, Banwell B, Villanova M, Fardeau M, Mandell JW, Juel VC, Rafii M, Auranen M, Kalimo H. Narrowing in on the causative defect of an intriguing X-linked myopathy with excessive autophagy. Neurology 2002 59:596-601.

Neuroscience Graduate Program

A more complete list of Dr. Mandell's publications can be obtained from PubMed