Summer Course 2007

Methods in Cell and Molecular Biology 

The Department of Microbiology offers a summer course that presents practical and theoretical aspects of selected basic methods of cell and molecular biology.

Participation in this course is open to all interested individuals, and entering graduate students are particularly encouraged to attend.

Coordinators:  Anjeanette Roberts and Julie Davis Turner

 Date  Day  Presenter(s)  Topic
 7/23  M  Amy Bouton

 Nucleic Acids and Expression Systems

  • Cloning Strategies, screening, expression systems
  • Gene/protein expression, mutagenesis
pdf file download presentation
 7/26  Th
 Jordan,7006		  Lucy
 Pemberton

 Functional Genomics

  • Protein-protein interacions, in vivo assays / screens
  • Systematic genomic analyses
 7/30  M
 MR-5, 1041
  Bob Nakamoto

 Protein Chemistry

  • Protein structure and folding, transmembrane topology
  • Techniques to elucidate structural information, ChIP 
 8/2  Th
 Jordan,7006		  Yongde Bao

 Some useful tools for genomic analyses

  • DNA Sequencing
  • Genotyping - SNP and STR
  • Microarray - and expanding platform
 8/6  M
 MR-5, 1041		  Deb Lannigan

 Functional Screening

  • Small molecule inhibitors
  • RNAi
 8/9

 Th
 Jordan,7006

 David Mullins

 Immunological Methods

  • Review of antibody characteristics
  • ELISA, Immunoprecipitation, Western blot 
 8/13  M
 Jordan,7006		  Joanne 
 Lannigan

 Flow Cytometry

  • Principles  of fluorochromes & flow cytometry
  • Applications for population and single-cell analyses
 8/16  Th
 Jordan,7006		  Ian Macara,
 Jay Brown

 Microscopic approaches

  • Fluorescence microscopy
  • Electron microscopy 
 8/20

 M
 Jordan,7006

 Wenhao Xu
 Tim Bender

 Transgenic animals

  • Principles of generating transgenic and knock-out mice
  • Inducible knock-outs