CIIC Service Request

Everyone must complete a service request form for CIIC services.  We prefer that requests come via the following online form or by the PDF form.

UVA researchers requesting services that require animal transport to Fontaine Research Park and protocol transfer to ACUC Protocol 3522, please complete the ACUC Animal Transfer & ACUC Protocol Transfer form (available in Word & PDF).  Instructions for animal transport can be found on the form or at UVA Comparative Medicine website.

Alternatively, follow this link to download the request form in PDF Format, fill out the requested information and email the completed file to us at islets@virginia.edu.

Contact Information
 

Please provide information about how to contact you if we have questions.

If you have questions please contact us 

Email: islets@virginia.edu or Phone: (434)243-4856 

DERC Member:

 

 Animal Information


Animal orders are placed following the UVA Center for Comparative Medicine (CCM) schedule. Animals orders MUST be placed AT LEAST ONE WEEK prior to requested date of service.  Animal deliveries may take longer than 1 week depending on CCM delivery schedule.  For more information please check the CCM website.

CIIC to provide animals:

 

 Pancreatic Islet Isolation

Isolation Species:
Culture conditions (samples Grouped or Individually cultured):

 

Histological/Tissue Preparation 

The DERC Cell & Islet core facility is able to procure tissue samples in preparation for histological staining protein, or RNA isolation in 4 ways:

  1. Submerse tissue in 4% PFA.
  2. Heart puncture and perfusion of animal with PBS followed by 4% PFA.
  3. Tissue frozen in liquid nitrogen after being placed in a conical tube, foil, or OCT freezing compound.
  4. RNALater or RNA denaturing solution provided by the customer.
Use this form to provide us an outline of needs. Please contact CIIC to provide specific instructions on tissue preparation.
Tissues to Harvest:
Tissue Preparation:

 

  Islet & Cell Function

Standard methods of islet function are available below, for more in depth or comprehensive assessment of islet function, please contact Dr. Craig Nunemaker via email or phone (434-924-0229).

 

Islet Calcium Imaging:
Glucose Stimulated Insulin Secretion (GSIS) by ELISA:

 

 

Thank you for filling out the DERC CIIC form for service.  Please note that we will try to accommodate all requests on the requested dates of service.  Upon receipt of this form, the CIIC will email the contact above to verify specific dates of service and specifics related to the services requested.