Recent Publications 

Elagib, K.E., Xiao, M., Hussaini, I.M., Delehanty, L.L., Palmer, L.L., Racke, F.K., Birrer, M.J.., Shanmugasundaram, G., McDevitt., M.A., Goldfarb, A.N.  Jun blockade of erythropoiesis: role for repression of GATA-1 by HERP2.   Mol Cell Biol. 2004 Sep;24(17): 7779-94. 

fig 9b

HERP2-transduced CD34+ cells show impaired erythroid differentiation.  Cells transduced with MSCV-HERP2-IRES-GFP were cultured in erythroid medium for 7 days, followed by flow cytometric analysis for the erythroid marker glycophorin A (GPA).

Delehanty., L.L., Mogass, M., Gonias, S.L., Racke, F.K., Johnstone, B. Goldfarb, A.N.  Stromal Inhibition of Megakaryocytic Differentiation is Associated with Blockade of Sustained Rap1 Activation.  Blood, 101: 1744-1751, 2003.

Figure

Stromal contact selectively blocks late-phase Rap1 activation.  K562 cells cultured with or without TPA and with or without HESS-5 stromal cell contact for the indicated durations were analyzed for Rap 1 and Ras activation as well as for Akt activation.
Elagib, K.E., Racke, F.K., Mogass, M., Khetawat, R., Delehanty, L.L., Goldfarb, A.N.  RUNX1 and GATA-1 coexpression and cooperation in megakaryocytic differentiation.  Blood. 2003 Jun 1; 101(11): 4333-41.

Figure 2

Expression profile of RUNX1, GATA-1, and CBF-beta in healthy human bone marrow. Top left panel, RUNX1; Top right panel, GATA-1; bottom panel, CBF-beta . Black arrows indicate megakaryocytes, and red arrows indicate erythroblasts. Immunoperoxidase staining was performed using avidin-biotin complex on paraffin-embedded marrow clot section. Slides were counterstained with Harris hematoxylin (original magnification x 400).