University of Virginia Health System

Skip Navigation
  • Health System
  • Calendars
  • Maps
  • A-Z Index
  • UVa
  • Flow Cytometry Core Facility
  • Equipment
  • Policies & Rates
  • Scheduling
  • Forms
  • Resources
    • Training
    • Protocols
    • Meetings/Seminars
    • Facility News
    • Useful Links
    • FlowJo Site License
    • Accessing/Mapping to the Flow Cytometry Server
  • FAQs
  • Contact
  • Search this Site
    View the Site Index

Protocols:

  • DNA Content for Cell Cycle Analysis of Fixed Cells With Propidium Iodide
    • Why do I need to count my cells?

  • Yeast cell cycle

  • Apoptosis Assays (Sub Diploid, Annexin V, TUNNEL, Caspase Activity)

  • Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques.

  • CFSE Labeling

  •  Intracellular Cytokine Staining 

  • Measurement of GFP expression & DNA content Using Paraformaldehye Fixation

  • 7-AAD staining of dead cells

  • Critical Aspects of Staining Cells for Flow Cytometry

  • Preparation of 2% Formaldehyde Solution

  • Fluorochrome Conjugation of Monoclonal Antibodies

  • Data Analysis Tutorial

  • AMS Instruction Manual

  • Staining Protocol for the Cell Permeant DNA Dye DRAQ5

  • Reducing Cellular Autofluorescence

  • Dead Cell Descrimination by EMA


Last modified on: May 29, 2008

  • Print this Page
  • E-mail this Page

Flow Cytometry Core Facility

PO Box 800734

Charlottesville, VA 22908

434-243-2695

fax: 434-982-1071

Maintained by flowcytometry@virginia.edu

© 2008 by the Rector and Visitors of the University of Virginia

Disclaimer | About this Site