Amnis Imagestream100TM
By combining microscopy and flow cytometry in a single platform, the ImageStream achieves a true breakthrough in cell analysis. Now the visual information that you obtain with a microscope can be fully integrated with the population statistics you would typically get from a flow cytometer. You can define and quantify cell subpopulations not only by signal intensity, but by the location of the signal in or on the cell. You can use cell and nuclear morphologies to characterize cell populations. In short, you can take full advantage of complex visual data to perform robust, multiparameter quantitative analyses.
In the ImageStream , cells are illuminated with both a brightfield source and 405nm, 488nm and 658nm lasers. The resulting composite images are spectrally decomposed into as many as 6 component images and captured using Time Delay Integration technology on a proprietary CCD camera. You see a brightfield, darkfield and up to 4 fluorescence images of every cell, while over 30 morphological and signal intensity features are calculated for every image - nearly 200 features in total. With the new Extended Depth of Field option the ImageStream depth of field to be is expanded more than 5-fold, enabling such applications as FISH In Suspension (FISHIS®), as well as many other resolution-sensitive applications.
High resolution images deliver quantitative information about cell structure and about the location of your target molecules. Follow translocation from the cytoplasm to the nucleus; quantify capping and co-capping reactions; separate cells based on unique morphologic criteria; study cell-cell interactions; differentiate cell states such as late apoptosis and necrosis -- the range of potential applications is extremely broad.
For more details on sample preparation and fluorochromes choices click here.
