Modified Becton Dickinson FACSCalibur Dual Laser

One (488nm and 637nm) benchtop cytometer (FACSCalibur A) capable of 7 parameter (five color) analysis.   This instrument  can detect three fluorochromes excited by the 488nm laser and two fluorochromes excited by the 637nm laser.

Examples of possible fluorochromes which can be evaluated on these instruments:

  • 488nm excitable: FITC, PE, PerCP, PE-Cy5, PE-Cy5.5, PE-Cy7, PerCP-Cy5.5, CFSE, GFP, Alexa 488, PI, 7-AAD, DRAQ 5, Acridine Orange, PKH-26, Fura Red, Fluo-3, DiI, DCFDA, Sytox Green, Cy3, Ethidium Bromide, DHR123, TMR, JC-1
  • 635 excitable (dual laser instrument only): APC, DRAQ 5, TO-PRO3, Cy5, Alexa 633, Alexa 647, APC-CY7 (FACSCalibur A only), and APC-Cy5.5 (FACSCalibur A only)

Emission filters on this instrument are fixed and are as follows:

  • Emissions from the 488nm excitation
    • FL1 - 530/30 nm (515-545 nm)
    • FL2 - 585/42 nm (564-606 nm)
    • FL3 - 670 nm LP (>670 nm)
  • Emissions from the 637nm excitation (FACSCalibur A)
    • FL4 - 667/27
    • FL5 - 765/20

Consult the facility for fluorochrome combination feasibility and necessary controls prior to designing your experiment. Located in Jordan Hall, Room 7051; 434-243-2711.

This instrument can be run with a 96 well plate automated sample acquisition system.

  • See Scheduling for information on scheduling time on this instrument.

amsgif  FACS Calibur