University of Virginia Health System

1993-Present Post-Doctoral Fellow, Cell Biology, Univ. of Virginia, Charlottesville, VA.
1992-1993 Post-Doctoral Fellow, Biochemistry and Molecular Biology, Univ. of Southern California, Los Angeles, CA.
1987-1992 Ph.D. in Biochemistry, Univ. of Southern California, Los Angeles, CA. Thesis: Control and Expression of Genes Involved in the Differentiation of Mesodermal Cell Lines.
1980-1983 M.S. in Biochemistry, Univ. of Montana, Missoula, MT. Thesis: Effect of Alkaline Phosphatase on the Function of Purified Reverse Transcriptase in Reconstructed Reverse Transcription
1979-1980 B.S. in Microbiology, Univ. of Montana, Missoula, MT.
1972-1976 B.A. in Biology (Minor in Chemistry), American International College, Springfield, MA.

P.A. Jones, M.J. Wolkowicz, M. Harrington and F. Gonzales. Methylation and expression of the MyoD1 determination gene. Philosophical Transactions of the Royal Society of London B 326: 277-284 (1990) .

P.A. Jones, M.J. Wolkowicz, W.M. Rideout, F. Gonzales, C.M. Marziasz, G.A. Coetzee and S.J. Tapscott. De Novo methylation of the MyoD1 CpG island during the establishment of immortal cell lines. Proceedings of the National Academy of Sciences USA 87: 6117-612 (1990) .

M.J. Wolkowicz, S.A. Coonrod, P.P. Reddi, J.L. Millan, M-C. Hofmann and J.C. Herr. Refinement of the differentiated phenotype of the spermatogenic cell line GC-2spd(ts). Biology of Reproduction 55: 923-932 (1996).

A. Mandal, S. Naaby-Hansen, M.J.Wolkowicz, K. Klotz, J. Shetty, S. Coonrod, M. Kinter, N. Sherman, F. Cesar, C.J. Flickinger and J.C. Herr. FSP95, a testis-specific, auto- and iso-antigenic 95kDa fibrous sheath protein undergoing tyrosine phosphorylation during human sperm capacitation. In preparation.

S. Naaby-Hansen, A. Mandal, M.J.Wolkowicz, C.J. Flickinger, and J.C. Herr. CBP86, a testis-specific calcium-binding protein is tyrosine phosphorylated during capacitation of the human sperm. In preparation.

M.J.Wolkowicz, S. Naaby-Hansen, C.J. Flickinger, and J.C. Herr. Cloning, Sequencing, Expression and Analysis of a Human Testicular Tektin B1-like Protein. In preparation.

M.J. Wolkowicz, L.A. Michalowsky and P.A. Jones. Characterization of a bipotential cell line derived from 10T1/2 cells Journal of Cellular Physiology (1996). Submitted (MS# 8424).

M.J. Wolkowicz and J.C. Herr. Cloning, mapping and sequencing of the upstream promoter region of SP-10, a testis-specific gene. In preparation.

M.J. Wolkowicz and P.A. Jones. Characterization of a bipotential cell line by subtractive hybridization. In preparation.

Coinventor- U.S. Patent #08/237,230; Continuation in Progress for: Peptide analogs of sperm fusion as contraceptives.

Coinventor- U.S. Patent Applied for 2-D Database and Process of Identifying Vaccine Immunogens from the Surface of a Biological Target.

Michael J. Wolkowicz and Kenneth F. Watson. Effect of Alkaline Phosphatase on the Reverse Transcriptase of Avian Myeloblastosis Virus During Reconstructed Transcription. Podium Talk at 43rd Annual Montana Academy of Sciences Meeting, Bozeman, MT, April, 1983. Proceedings of Montana Academy of Sciences 43:16 (1983).

Michael J. Wolkowicz and Peter A. Jones. Isolation by Subtractive Hybridization of Genes Expressed in a Bipotential Cell Line. Poster at 83rd Annual A.A.C.R Meeting, San Diego, CA, May, 1992. Proceedings of American Association for Cancer Research 33: 358 (1992).

Michael J. Wolkowicz and John C. Herr. Tissue-Specific Gene Expression During Acrosomal Biogenesis. Podium Talk at 14th Annual A.S.R.I. Meeting, Philadelphia, PA, June, 1994. American Journal of Reproductive Immunology 31(4): 225 (1994).

Michael J. Wolkowicz, P.P. Reddi, A.J. Freemerman, C.J. Flickinger and J.C. Herr. SP-10: A Model Testis-Specific Antigen. Podium Talk at 16th International Congress of Biochemistry and Molecular Biology, New Delhi, India, September, 1994. Proc. of I.C.B.M.B. 16(III): 37 (1994).

Michael J. Wolkowicz and J.C. Herr. Analysis of the GC-2spd(ts) cell line for expression of cell-type specific markers of differentiation. 35th Meeting of A.S.C.B., Washington, DC, December, 1995. Molecular Biology of the Cell (Supplement) 6: 315a (1995).

Herr, J.C., Nabby-Hansen, S., Diekman, A.B., Wolkowicz, M.J., Westbrook-Case, A., Norton, E., Klotz, K., Shibahara, H., Koul, D., Mandal, A., Shetty, J. and Flickinger, C.J.Discovery and Characterization of Sperm Contraceptive Vaccinogens. CSIRO, Australia (1997).

Nabby-Hansen, S., Wolkowicz, M.J., Shibahara, H., Shetty, J., C.J. Flickinger and J.C. Herr.Towards a comprehensive 2-D gel protein database for the human spermatozoon. 30th Annual Meeting of the Society for the Study of Reproduction. Portland, Oregon. Biology of Reproduction, 56(Supplement 1):172 (Abs. 358) (1997).

Nabby-Hansen, S., Wolkowicz, M.J., Shannon, J., Kinter, M., Reddy, P.P., Shibahara, H., Bush, L.A., Fox, J. and Herr, J.C. Human spermatozoa calreticulin: From 2-D gel spot to isolation of testicular cDNA. 13th Ann. Meeting ESHRE, Edinburgh (1997).

Herr, J.C., Nabby-Hansen, S., Wolkowicz, M.J., Mandal, A., Jagatpalla, S. and Flinkinger, C.J. A strategy for identification and cloning of novel sperm surface immunogens. Experimental Biology (1998).

Wolkowicz, M.J., Nabby-Hansen, S. and Herr, J.C. Cloning, Sequencing, Expression and Analysis of a Human Testicular Tektin B1-like Protein. 31st Ann. Meeting Society for the Study of Reproduction. Biology of Reproduction 58(Supplement 1):175 (Abs. 331) (1998).

Mandal, A., Naaby-Hansen, S., Klotz, K., Wolkowicz, M.J., Shetty, J., Cesar, F., Flickinger, C.J. and Herr, J.C. Two novel human sperm fibrous sheath proteins which undergo phosphorylation during capacitation. ASCB Meeting.