Charles J. Flickinger

Associate Director of CRCRH;
Director of Tissue Specificity Core;
Professor and Chair, Dept. of Cell Biology

CRCRH,
Department of Cell Biology
University of Virginia
Health System Box 800732
Charlottesville, VA 22908

(434) 924-9979
cjf@virginia.edu
http://www.med.virginia.edu/medicine/basic-sci/cellbio/cjf.htm

Education

Dartmouth College, A.B., Chemistry, 1960
Harvard Medical School, M.D., 1964

Research
My laboratory is, among other studies, involved in morphological, biochemical, and molecular studies of human sperm antigens, in collaboration with Dr. John Herr. We are using immunocytochemistry to localize antigens in spermatozoa and in the testis, and we are using northern analysis, RT-PCR, and immunocytochemistry to perform tissue specificity studies of putative gamete-specific antigens. We have also studied testicular gene expression with in situ hybridization methods to detect specific germ cell mRNAs.

Representative Publications

Flickinger,C.J., Rao,J., Bush,L.A., Sherman,N., Oko,R., and Herr,J.C. (2001). Outer dense fiber proteins are dominant post-obstruction autoantigens in adult Lewis rats. Biol. Reprod. 64, 1459.

Flickinger,C.J., Vagnetti,M., Howards,S.S., and Herr,J.C. (2000). Antisperm autoantibody response is reduced by early repair of a severed vas deferens in the juvenile rat. Fertil. Steril. 73, 229-237.

Flickinger,C.J., Bush,L.A., Williams,M.V., Naaby-Hansen,S., Howards,S.S., and Herr,J.C. (1999). Post-obstruction rat sperm autoantigens identified by two-dimensional gel electrophoresis and western blotting. J. Reprod. Immunol. 43, 35-53.

Herr,J.C., Thomas,D., Bush,L.A., Coonrod,S., Khole,V., Howards,S.S., and Flickinger,C.J. (1999). Sperm mitochondria associated cysteine-rich protein (SMCP) is an autoantigen in Lewis rats. Biol. Reprod. 61, 428-435.

Naaby-Hansen,S., Flickinger,C.J., and Herr,J.C. (1997). Two-dimensional gel electrophoretic analysis of vectorially labeled surface proteins of human spermatozoa. Biol. Reprod. 56, 771-787.