Articles & Publications

cvr jnl051503


Journal of Neurobiology

The Journal of Neuroscience, Volume 23, Number 10, May 15, 2003

We describe a novel mechanism for vital fluorescent dye entry into sensory cells and neurons: permeation through ion channels. In addition to the slow conventional uptake of styryl dyes by endocytosis, small styryl dyes such as FM1-43 rapidly and specifically label hair cells in the inner ear by entering through open mechanotransduction channels.

 

The Journal of Neurobiology, Volume 50, Number 2, February 5, 2002

 A scanning electron micrograph of the surface of a bullfrog’s saccule that shows a hair bundle and cuticular plate that have separated from the soma of a gentamicin-damaged hair cell.  Exposure to short-term, low-dose gentamicin causes loss of the sensory apparatus from hair cells, which can survive and recover.  Electron microscopy, time-lapse, and multi-photon recordings were used to observe bundle separation, survival of bundleless hair cells, and repair of damaged hair cell surfaces as described in Gale et. al.

 

Journal of Neuroscience The Journal of Neuroscience, Volume 21, Number 2, January 15, 2001

 Glial growth factor (GGF2) strongly stimulates S-phase entry in the early postnatal rat utricle.  DIC micrograph of a piece of rat utricular sensory epithelium composed only of supporting and hair cells, and cultured for 72 hr in a medium containing GGF2.  Nuclei of cells that entered S-phase have incorporated BrdU (red).  Fluorescent DAPI staining revealed the nuclei that did not enter S-phase in the same piece of epithelium (green).  For details, see the article by Montcouquiol and Corwin in this issue (pages 570-580).

 

JARO JARO Volume 1, Number 2, September 2000

 Extramacular hair cell from the bullfrog saccule, labeled with HCS1, a specific hair cell antibody, in red.  Green is phalloidin label.  For a detailed description, see the paper by Gales, Meyers, and Corwin in this issue.

 

Selected Recent Publications

  1. Corwin, J. and Cotanche, D.  1988.  Regeneration of sensory hair cells after acoustic trauma.  Science, 240: 1772-1774.
  2. Forge, A., Li, L., Corwin, J., and Nevill, G. (1993) Ultrastructural evidence for hair cell regeneration in the mammalian inner ear. Science 259:1616-1619.
  3. Warchol, M., Lambert, P., Goldstein, B., Forge, A., and Corwin, J. (1993) Regenerative proliferation in inner ear sensory epithelia from adult guinea pigs and humans. Science 259:1619-1622.
  4. Kelley, M., Xu, X., Wagner, M., Warchol, M., and Corwin, J. (1993) The developing organ of cortin contains retinoic acid and forms supernumerary hair cells in response to exogenous retinoic acid in culture. Development 119:1041-1053.
  5. Warchol, M. and Corwin, J. (1993) Supporting cells in avian vestibular organs proliferate in serum-free culture. Hear Res. 71:28-36.
  6. Kelley, M., Talreja, D. and Corwin, J. (1995) Replacement of haircells after laser microbeam irradiation in cultured organs of Corti from embryonic neonatal mice. J. Neurosci. 15:3013-3026.
  7. Warchol, M. and Corwin, J. (1996) Regenerative proliferation in organ cultures of the avian cochlea: Identification of the initial progenitors and determination of the latency of the proliferative response. J. Neurosci. 16: 5466-5477.
  8. Jones, J. and Corwin, J. (1996) Regeneration of sensory cells after laser ablation in the lateral line system: Hair cell lineage and macrophage behavior revealed by time-lapse video microscopy. J. Neurosci. 16: 649-662
  9. Saffer, L., Gu, R. and Corwin, J. (1996) An RT-PCR analysis of mRNA for growth factor receptors in damaged and control sensory epithelia of rat utricles. Hearing Res. 94: 14-23.
  10. Corwin, J., Warchol, M., Saffer, L., Finley, J., Gu, R., and Lambert, P. (1996) Growth factors as potential drugs for the sensory epithelia of the ear. In: Growth factors as drugs for neurological and sensory disorders. Ciba Foundation Symposium 1996. John Wiley and Sons, Chichester (pp. 167-187).
  11. Warchol, M.E. and Corwin, J.T. (1996) Regenerative proliferation in organ cultures of the avian cochlea: Identification of the initial progenitors and determination of the latency of the proliferative response. 
    J. Neurosci. 16: 5466-5477.
  12. Kil, J. Warchol, M.E. and Corwin, J.T. (1997) Cell death, cell proliferation, and estimates of hair cell life spans in the vestibular organs of chicks. Hearing Res. 114: 117-126
  13. Corwin, J.T. (1997) (Guest Editor of this volume) Development of the Ear. Seminars in Cell and Developmental Biology, Academic Press 8: 215-216.
  14. Oberholtzer, J. Carl and Corwin, J.T. (1997) Fish 'n Chicks: Model Recipes for Hair Cell Regeneration? Neuron (invited minireview) 19: 951-954.
  15. Gale, J., Meyers, J. R., and Corwin, J. T. 2000. Solitary hair cells are distributed throughout the Extramacular Epithelium in the Bullfrog's Saccule. J.A.R.O. 1:172-182.
  16. Montcouquiol, M. and Corwin, J. T. 2001a. Intracellular Signals that control Cell Proliferation in Mammalian Balance Epithelia: Key Roles for PI 3-kinase, mTOR, and S6 Kinases in preference to Calcium, PKC, and MAPK. J. Neurosci. 21:570-580.
  17. Montcouquiol, M. and Corwin, J. T. 2001b. Brief treatments with forskolin enhance S-phase entry in balance epithelia from rodents.
    J. Neurosci. 21:974-982.
  18. Witte, M., Montcouquiol, M., and Corwin, J. T. 2001. Regeneration in avian hair cell epithelia: Indentification of Intracellular Signals Required for S-phase Entry. Eur. J. Neurosci. 14:829-838.
  19. Gale, J. E., Meyers, J. R., Periasamy, A., and Corwin, J. T. 2002. Survival of bundle-less hair cells and subsequent bundle replacement in the bullfrog saccule. J. Neurobiol. 50:81-92.
  20. Meyers, J.R., MacDonald, R.B., Duggan, A., Lenzi, D., Corwin, J.T., and Corey, D.P. 2003. Lighting up the senses: FM1-43 loading of sensory cells through non-selective ion channels. J Neurosci. 23: 4054-4065.