FGFs, Hox genes and cell differentiation

The Lui laboratory studies the molecular mechanisms that control the development of the central nervous system. Techniques in molecular biology, cell biology, and embryology were used to identify molecules that control the rosctrocaudal patterning of the developing spinal cord. Many genes that control growth and differentiation during embryonic development are also involved in cancer formation/progression. Understanding the mechanisms that regulate the expression of these genes will benefit cancer research and cancer treatment in the long run.

A major focus of the laboratory is on the molecular control of Hox gene expression and the functions of Hox genes in determining neuronal identities and projections. Using an in vitro assay system, fibroblast growth factor 8 (FGF8), growth and differentiation factor 11 (GDF11) and the caudal-related family of transcription factors (Cdx) were identified as key players in determining neural Hox gene expression domains. Unregulated expression of FGF, Cdx, and Hox genes has been implicated in various cancers.

Currently, transgenic mice and chick in ovo electroporation are being used to evaluate the in vivo functions and connections between these molecules. DNA microarray analyses are being used to identify the targets of Hox genes. The DNA science Core plays a major role in this research. The Hybridoma Core has also provided valuable assistance in generating an anti-Cdx antibody.