Rates

Rates current as of May 2009 are shown.

Payment from outside the University of Virginia can be made with a credit card, check or wire transfer. See instructions on the web site of the Office of Research Core Administration
http://www.healthsystem.virginia.edu/internet/orca/outside_billing.cfm

 Approved grants receive a co-payment from the Cancer Center. The co-payment is automatically applied through the CORES billing system.

DNA Sequencing

 

University of Virginia

 Outside rate

    1-16 reactions  $7.50  $11.40
    16-48 reactions  $7.00 

 $10.64

    48+ reactions   $6.70  $10.18 
 Full plate sequencing (96 reactions)   $460.80  $700.42
 Primer walking sequencing    
   Project based on length,per kb   $88.00   $133.76
   primers as needed for primer walking   $8.00 setup + $0.75 per base   
  

Affymetrix GeneChip Expression Analysis

  
 

 University of Virginia

outside rate 

 Starting material of total RNA    
   full GeneChip analysis service, including
   data analysis 		 $375.00  $570.00 
   2 cycle GeneChip analysis   $550.00   $836.00 
   Total RNA isolation from cells, per sample   $38.00   $58.00 
   Total RNA isolation from tissue, per sample   $45.00  $68.00 
 Starting material of labeled cRNA
   hybridization and scanning  $75.00   $114.00
   RNA quality check by Agilent Bioanalyzer, per well   $10.00   $15.20 
   RNA quality check by Agilent Bioananalyzer,
   per cartridge  		 $72.00   $109.44
 Affymetrix GeneChips; prices vary by chip type   from $250 to $425  from $250 to $425 
   Extended analysis; genes showing 
   differential expression, clustering etc. 		 $75/hr   
 Consultation  $75/hr   
     

Real time PCR

 

University of Virginia

Outside rate

 cDNA synthesis/sample for RT-PCR,
50 µL scale, per RNA  1-16 samples		  $12.00  $18.24
 cDNA synthesis/sample for RT-PCR,
 50 µL scale, per RNA  >16 samples		  $10.80  $16.42
 Quantitative real time PCR, 1-48 /gene/sample  $9.00

 $13.68

 Quantitative real time PCR, >48 /gene/sample  $8.10  $12.31 
 Equipment use for real time PCR, per hour   $30.00   $45.60
 Equipment use for end point post PCR read (SNP assay)   $24.00  $36.48
 Primer-probe design and consultation, per hour   $75.00   $114.00

Oligonucleotide Synthesis

contact MolMart (email: smd-mol@virginia.edu ) 4-9211 to order through Invitrogen Supply Center

 

SNP Genotyping  

  University of Virginia  Outside rate
 Assay Design  $20/SNP   $30.40/SNP 
 Data analysis, no PCR  $145 per 96 well plate  $220 per 96 well plate 
 PCR and data analysis  $195 per 96 well plate   $296 per 96 well plate
  

  

Mass Spectrometry

   University of Virginia  outside University of Virginia
 MALDI MS  $50  $155
 High accuracy mass determination (ESI-FT-ICR)  $250  $379
 Electrospray with analysis  $300  $444
 Peptide Mass mapping  $250  $379
 Sequencing project  $500  $732
 Analysis of Complex mixture  $1,250  $6,287
 Post translational modifications or de novo sequencing  $1,000  $6,060

Peptide synthesis

Peptides are now ordered from Anaspec through Molmart  

HPLC, protein interactions, gel scanning and shared instrumentation

   University of Virginia  outside University of Virginia
 HPLC/FPLC setup for one or more samples  $48  $120 
 HPLC/FPLC per injection, no collection  $46  $112
 HPLC/FPLC per injection, collection of fractions  $60   $148 
 HPLC/FPLC method development  $311   $471
 purification of synthetic peptide  $200   $303 
 protein digestion or modification  $90  $136
 scan gel (densitometry or fluorescence)  $25  $61
 Biacore 3000 instrument use  $20/hr  $61/hr
 Protein interaction analysis  $75/hr  $132/hr

User operated Instruments

 Service  University of Virginia  outside users
 Fluorescent plate reader, first 10 minutes  $12  $36
 Fluorescent plate reader, per hour after first 10 minutes  $16  $24
 Create new account for fluorescent plate reader and introductory training  $25  $95
 circular dichroism spectropolarimeter  $30/hr  $73/hr
 preparative HPLC with user supplied column  $30/hr  $45/hr