Rates
Rates current as of December 2007 are shown.
Payment from outside the University of Virginia can be made with a credit card by going to: http://www.web.virginia.edu/CORES/creditcard.htm
Approved grants receive a co-payment from the Cancer Center. The co-payment is automatically applied through the CORES billing system.
DNA Sequencing
|
|
University of Virginia |
Outside rate |
| 1-16 reactions | $9.60 | $14.59 |
| 16-48 reactions | $9.00 | $13.68 |
| 48+ reactions | $8.16 | $12.40 |
| Full plate sequencing (96 reactions) | $487.00 | $740.24 |
| Primer walking sequencing | ||
| Project based on length,per kb | $88.00 | $133.76 |
| primers as needed for primer walking | $8.00 setup + $0.75 per base |
Affymetrix GeneChip Expression Analysis
|
University of Virginia |
outside rate |
|
| Starting material of total RNA | ||
| full GeneChip analysis service, including data analysis |
$375 | $570 |
| 2 cycle GeneChip analysis | $550 | $836 |
| Total RNA isolation from cells, per sample | $38 | $58 |
| Total RNA isolation from tissue, per sample | $45 | $68 |
| Starting material of labeled cRNA | $220 | $334 |
| hybridization and scanning | $72 | $109 |
| RNA quality check by Agilent Bioanalyzer, per well | $10 | $14 |
| RNA quality check by Agilent Bioananalyzer, per cartridge |
$72 | $109 |
| Affymetrix GeneChips; prices vary by chip type | from $250 to $425 | from $250 to $425 |
| Extended analysis; genes showing differential expression, clustering etc. |
$75/hr | |
| Consultation | $75/hr |
Printed arrays
| University of Virginia | outside rate | |
| mouse cytokines array, per slide | $81 | $123.12 |
| human cytokines array, per slide | $81 | $123.12 |
| target labeling, hydridization and data analysis starting from RNA, for 2 paired samples | $216 | $328.32 |
| target hydridization, scan and data analysis | $85 | $129.20 |
| Imaging | ||
| ScanArray slide imaging to tiff file | $6/channel/array | $9/channel/array |
| Analysis | ||
| standard analysis: Axon GenePix Spreadsheet: raw and global normalized analysis per array |
$75/hr | $114/hr |
| extended analysis: genes showing differential expression | $75/hr | $114/hr |
| Consultation | $75/hr | $114/hr |
Real time PCR
|
|
University of Virginia |
Outside rate |
| cDNA synthesis/sample for RT-PCR, 50 µL scale, per RNA |
$12 | $18 |
| real time run, each well | $3 | $5 |
| equipment use for real time PCR, per hour | $30 | $46 |
| equipment use for end point post PCR read (SNP assay) | $24 | $36 |
| primer-probe design and consultation, per hour | $75 | $114 |
Oligonucleotide Synthesis
| University of Virginia | Outside Rate | |
| 50 nmole | ||
| startup | $6 | $9.12 |
| each base | $0.60 | $0.91 |
| 0.2 µmole | ||
| startup | $6.00 | $9.12 |
| each base | $0.90 | $1.37 |
| 1 µmole | ||
| startup | $10.50 | $15.96 |
| each base | $1.50 | $2.28 |
| Desalting | $12.00 | $18.24 |
| Purification by cartridge | $16.50 | $25.08 |
| Purification by ion exchange chromatography | $45.00 | $68.40 |
SNP Genotyping
| University of Virginia | Outside rate | |
| Assay Design | $20/SNP | $30.40/SNP |
| Data analysis, no PCR | $145 per 96 well plate | $220 per 96 well plate |
| PCR and data analysis | $195 per 96 well plate | $296 per 96 well plate |
Mass Spectrometry
| base rate | School of Medicine rate | outside University of Virginia | |
| MALDI MS | $102 | $50 | $155 |
| High accuracy mass determination (ESI-FT-ICR) | $250 | $250 | $379 |
| Electrospray with analysis | $300 | $300 | $444 |
| Peptide Mass mapping | $250 | $250 | $379 |
| Sequencing project | $500 | $500 | $732 |
| Analysis of Complex mixture | $4,150 | $1,250 | $6,287 |
| post translational modifications or de novo sequencing | $4,000 | $1,000 | $6,060 |
| biomarker sample | $4,760 | $2,240 | $7,211 |
Peptide synthesis
| University of Virginia | outside University of Virginia | University of Virginia | outside University of Virginia | University of Virginia | outside University of Virginia | |
| 25 µmole synthesis | 25 µmole synthesis | 50 µmole synthesis | 50 µmole synthesis | 100 µmole synthesis | 100 µmole synthesis | |
| first 15 amino acids | $265 | $723 | $337 | $767 | $540 | $1001 |
| each additional amino acid | $18 | $53 | $30 | $56 | $36 | $67 |
Other synthetic peptide services
|
University of Virginia |
outside University of Virginia | |
|
custom purification of non-BRF peptide |
$200 | $303 |
| conjugation of 2 mg of peptide to 2 mg of carrier protein | $230 | $348 |
| conjugation of 10 mg of peptide to 10 mg of protein | $421 | $638 |
Protein sequencing by Edman chemistry
| University of Virginia | outside University of Virginia | |
| startup | $125 | $301 |
| per amino acid (minimum 1) | $35 | $77 |
HPLC, amino acid analysis, protein interactions and other protein chemistry services
| University of Virginia | outside University of Virginia | |
| amino acid analysis, first sample | $110 | $197 |
| amino acid analysis, additional sample | $80 | $173 |
| HPLC/FPLC setup for one or more samples | $48 | $120 |
| HPLC/FPLC per injection, no collection | $46 | $112 |
| HPLC/FPLC per injection, collection of fractions | $60 | $148 |
| HPLC/FPLC method development | $311 | $471 |
| purification of synthetic peptide | $200 | $303 |
| protein digestion or modification | $90 | $136 |
| scan gel (densitometry or fluorescence) | $25 | $61 |
| Biacore 3000 instrument use | $20/hr | $61/hr |
| Protein interaction analysis | $75/hr | $132/hr |
User operated Instruments
| Service | University of Virginia | outside users |
| Fluorescent plate reader, first 10 minutes | $12 | $36 |
| Fluorescent plate reader, per hour after first 10 minutes | $16 | $24 |
| Create new account for fluorescent plate reader and introductory training | $25 | $95 |
| circular dichroism spectropolarimeter | $30/hr | $73/hr |
| preparative HPLC with user supplied column | $30/hr | $45/hr |