Shared Instrumentation Core

The Shared Instrumentation Core within the Biomolecular Research Facility has several user and staff operated instruments and services, largely for studying proteins.  These instruments and services and some discontinued services formerly constituted the Protein Sciences Core.

Fluorescent plate reader for reading fluorescent reagents in 96 well plates

Circular dichroism for study of secondary structure of proteins.

Chromatography by HPLC or FPLC of proteins and peptides and other molecules

Gel scanning for visible and fluorescent stains

A spot cutter for cutting spots from gel stained with Sypro Ruby and visible stains (staff operated).

Dynamic light scattering instrument (user operated).

The Biomolecular Research Facility has discontinued N-terminal sequencing using Edman chemistry;searches at http://www.abrf.org/index.cfm/dir.yp and elsewhere on the web will find other facilities which perform this analysis.

Peptide synthesis is now performed by Anaspec; order through Molmart

Protocols

Methanol chloroform precipitation of proteins. This technique has been used to concentrate and desalt proteins.